K-State home » Integrated Genomics  » Equipment » -CFX 96 Real-Time PCR
Integrated Genomics

The CFX 96 Real-Time PCR Detection System (Bio-Rad)

   equipment>CFX 96 (Bio-Rad)


                  KASP protocol (LGC Genomics)





  • The CFX 96 Real-Time PCR System build on Bio-Rad's proven 1000-series thermal cycling platform. Fast thermal cycling produces results in less than 30 minutes.
  • The system incorporates innovated optical technologies with long-lasting LEDs.
  • The CFX 96 is factory-calibrated, no further calibration will require.
  • It allows up to 5-target multiplexing.
  • The reaction volume can be as low as 10ul.
  • It includes thermal gradient feature, so the assay optimization could be done in a single run.
  • At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to normalize to a passive reference dye.
  • CFX Manager software can be used not only to visualize all run data at once, but also to perform the complete gene expression analysis.
  • The system sends an automatic email notification with an attached data file when a run is finished.
  • The CFX 96 with its excellent thermal stability and sensitivity and high-resolution melt analysis dedicated software can be used for detection single-base sequence variations or to discover unknown genetic mutations. High-resolution melt curve analysis can be considered the next-generation application of amplicon melting analysis. 



Equipment Producer:

Useful Information for Real-Time PCR: