Real-Time PCR Workshop
June 19 - 20th, 2013
The IGF offers the two-day Real-Time PCR workshop. The workshop is a combination of lectures and hands-on experiments on the Real-Time PCR, primer/probe design, experimentation, and data analysis. Participants will perform SYBR Green I based gene expression analysis, TaqMan probe based diagnostic analysis and KASP assay based allelic discrimination analysis on provided samples using the Bio-Rad CFX 96 Real-Time PCR system. This will simulate the complete process of Real-Time PCR reactions and analysis of subsequent data.
Overview of Real-Time PCR Technology
A practical approach to assay design for qPCR
Real-Time PCR for plant pathogen diagnostics
Lab / Computer lab Exercise
Primer and probe design
Gene expression analysis reaction set up
KASP genotyping assay reaction set up
TaqMan plant pathogen diagnostics assay reaction set up
There is no registration fee, but the registration is limited to 16 attendees, and is accepted on first-come, first-serve basis. To register please contact to Alina Akhunova by email firstname.lastname@example.org or by phone 785-532-1393
Course Credit Option (Tuition Fee)
PLPTH 785 (Course number 10579), Real-Time PCR workshop, one graduate credit. Rec. Pr.: one of the following courses: PLPTH 610, AGRON 610, BIOCH 521, BIOCH 522, BIOL 675, BIOL 676.