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Integrated Genomics

Real-Time PCR Workshop

June 19 - 20th, 2013

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The IGF offers the two-day Real-Time PCR workshop. The workshop is a combination of lectures and hands-on experiments on the Real-Time PCR, primer/probe design, experimentation, and data analysis. Participants will perform SYBR Green I based gene expression analysis, TaqMan probe based diagnostic analysis and KASP assay based allelic discrimination analysis on provided samples using the Bio-Rad CFX 96 Real-Time PCR system. This will simulate the complete process of Real-Time PCR reactions and analysis of subsequent data. 

Lectures

  • Overview of Real-Time PCR Technology
  • A practical approach to assay design for qPCR
  • Real-Time PCR for plant pathogen diagnostics

Lab / Computer lab Exercise

  • Primer and probe design
  • Protocol programming
  • Gene expression analysis reaction set up
  • KASP genotyping assay reaction set up
  • TaqMan plant pathogen diagnostics assay reaction set up
  • Data analysis

Registration

There is no registration fee, but the registration is limited to 16 attendees, and is accepted on first-come, first-serve basis. To register please contact to Alina Akhunova by email akhunova@ksu.edu or by phone 785-532-1393 

Course Credit Option (Tuition Fee)
PLPTH 785 (Course number 10579), Real-Time PCR workshop, one graduate credit. Rec. Pr.: one of the following courses: PLPTH 610, AGRON 610, BIOCH 521, BIOCH 522, BIOL 675, BIOL 676.